| 中文名称 | 过敏毒素C3受体/补体C3受体抗体 |
| 别 名 | AZ 3B; AZ3B; C3a anaphylatoxin chemotactic receptor; C3A R; C3A R1; C3a-R; C3AR; C3AR_HUMAN; C3AR1; C3R1; Complement component 3 receptor 1; Complement component 3a receptor 1; HNFAG09. |
| 研究领域 | 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 54kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human C3a Receptor/C3aR:401-482/482 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Complement C3 precursor contains complement C3 beta chain, complement C3 alpha chain, C3a anaphylatoxin, complement C3b alpha chain, complement C3c fragment, complement C3dg fragment, complement C3g fragment, complement C3d fragment and complement C3f fragment. C3a, C4a, and C5a are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage that aids in the elimination of pathogens. C3a and C5a secretion correlates with pathophysiological phenotypes such as asthma and bacterial meningitis. Binding of these proteins to their respective G protein-coupled receptors (C3aR, C5aR), which are present on the surface of myeloid leukocytes, induces proinflammatory events such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. C3aR is expressed in brain and activated B-lymphocytes whereas C5aR is prevalent on the surface of hepatocyte, lung, smooth muscle, and endothelial cells. Upon activation, C3aR and C5aR are susceptible to rapid GRK-mediated phosphorylation and clathrin-coated vesicle targeting. C5aR utilizes the Ras-Raf-ERK1/2 cascade and couples to Gi/G16 proteins. Function: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues. Post-translational modifications: Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q16581 Gene ID: 719 Database links: Entrez Gene: 719 Human Entrez Gene: 12267 Mouse Entrez Gene: 84007 Rat Omim: 605246 Human SwissProt: Q16581 Human SwissProt: O09047 Mouse SwissProt: O55197 Rat Unigene: 591148 Human Unigene: 2408 Mouse Unigene: 9772 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample:K562(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Lung (Mouse) Lysate at 40 ug Primary: Anti-C3a Receptor (bs-2955R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 64 kD  Sample: 293T Cell (Human) Lysate at 30 ugPrimary: Anti-C3a Receptor (bs-2955R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 60kD  Sample: A549 Cell (Human) Lysate at 30 ugPrimary: Anti-C3a Receptor (bs-2955R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 60kD  Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C3a Receptor) Polyclonal Antibody, Unconjugated (bs-2955R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |