Nature Commun | 福建农林大学许卫锋团队与中国农科院程锋团队发布白羽扇豆高质量基因组，解析低磷适应机制

研究使用平台：NMT磷营养创新科研平台

期刊：Nature Communications

主题：质子流在白羽扇豆基因组进化和低磷适应研究上的关键作用

标题：The genome evolution and low-phosphorus adaptation in white lupin

影响因子：11.878

检测指标：H+流速

检测样品：白羽扇豆

H+流实验处理方法：

检测簇状根和正常根

H+流实验测试液成份：

0.1mM KCl，0.1mM CaCl2、0.1mM MgCl2、0.5mM NaCl，0.3mM MES，0.2mM Na2SO4，pH 6.0

作者：福建农林大学许卫锋、张仟、夏天雨；中国农业科学院蔬菜花卉研究所程锋

中文摘要（谷歌机翻）

白羽扇豆（羽扇豆（Lupinus albus））是一种豆科植物，可生长簇状根，在低磷土壤中具有较高的磷（P）利用效率（PUE）。

在这里，我们组装了白羽扇豆的基因组，发现它是从全基因组三倍（WGT）事件演变而来的。然后，我们破译其二倍体祖先基因组并重建三个亚基因组。

根据结果，我们进一步揭示了不同亚基因组的亚基因组优势和基因表达，这些基因组的表达随其转座子（TE）密度而变化。白羽扇豆中的PUE基因已通过WGT以及串联和分散重复进行了扩增。

此外，我们表征了高PUE的四个主要途径，包括碳固定，簇根形成，土壤P固定和细胞P重用。其中，生长素调节可能通过参与LaABCG37s对簇根形成很重要。这些发现为白羽扇豆的基因组进化和低磷适应提供了见识。

（b）

（c）

( b ) Hydrolytic activity and H+ flux rate of PM H+-ATPase in different types of roots. n = 3 plants for PM ATPaseactivity and 4 plants for H+ flux . ( c ) Effects of vanadate ( Na3VO4 , inhibitor of P-type ATPase ) on H+ flux rate and cluster root formation in white lupin under P sufficient and deficient condition. n = 4 plants . Error bars indicate s.e. m., P value was calculated using the unpaired two-sided Student's t-test . + PR , P sufficient roots ; - PNR , P deficientmomal roots ; -PCR P deficient cluster roots ; Lane 1 . + PR ; Lane 2 , - PNR ; Lane 3，PCR . Source data are provided as a Source Data file.

英文摘要

White lupin (Lupinus albus) is a legume crop that develops cluster roots and has high phosphorus (P)-use efficiency (PUE) in low-P soils.

Here, we assemble the genome of white lupin and find that it has evolved from a whole-genome triplication (WGT) event. We then decipher its diploid ancestral genome and reconstruct the three sub genomes.

Based on the results, we further reveal the sub-genome dominance and the genic expression of the different sub-genomes varying in relation to their transposable element (TE) density. The PUE genes in white lupin have been expanded through WGT as well as tandem and dispersed duplications.

Furthermore, we characterize four main pathways for high PUE, which include carbon fixation, cluster root formation, soil-P remobilization, and cellular-P reuse. Among these, auxin modulation may be important for cluster root formation through involvement of LaABCG37s. These findings provide insights into the genome evolution and low-P adaptation of white lupin.