外周蛋白即用型免疫组化试剂盒-分析方法-资讯-生物在线

外周蛋白即用型免疫组化试剂盒

作者:上海信裕生物科技有限公司 2023-04-25T00:00 (访问量:246)

 外周蛋白即用型免疫组化试剂盒

英文名称 Peripherin Ready-To-Use IHC Kit
中文名称 外周蛋白即用型免疫组化试剂盒
别    名 NEF 4; NEF4; NEF-4; Neurofilament 4; PRPH; PRPH1; PRPH 1; NEF 4; Neurofilament 4 (57kD); Perf; PERI_HUMAN.   
种    属 Human, Mouse, Rat
应用范围 Immunohistochemistry
适用样品基质 PFPE tissue
描述 IHC0108 is a ready-to-use IHC kit for staining of Peripherin. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
保存条件 Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍

产品组分及规格

编号

组分

规格

浓度

储存

1

PBS缓冲液干粉

2 L×2

20x

室温

2

抗原修复缓冲液

20 ml

100x

2-8

3

内源性过氧化物酶阻断剂

3 ml

RTU

2-8

4

封闭工作液

3 ml

RTU

2-8

5

一抗Peripherin Rabbit pAb

6 ml

RTU

2-8

6

二抗HRP-Goat anti-Rabbit IgG pAb

6 ml

RTU

2-8

7

DAB kit20×)显色液

0.3 ml

RTU

-20℃

8

DAB kit20×)稀释液

0.3 ml

RTU

-20

9

复染试剂

5 ml

RTU

室温

10

封片剂

5 ml

RTU

室温

11

对照大鼠结肠,小鼠结肠,人结肠

3

RTU

室温

12

说明书

1

 

 

 

背景

Peripherin is a 57kD type III intermediate filament that is a specific marker for peripheral neurons, including enteric ganglion cells. Peripherin is expressed in the developing peripheral nervous system and is highly enriched in neuronal derivatives of the neural crest. Peripherin offers an advantage over other neural markers, such as S100 and NSE in that it does not stain chrmaffin cell types.

 

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次,每次15 min;依次置于不同浓度(100%95%90%80%70%)乙醇浸泡5 min,再置于蒸馏水洗涤5 min,重复3次。

2. 抗原修复

沸水浴修复:将100×抗原修复缓冲液(试剂2用蒸馏水稀释成抗原修复缓冲液,放入修复盒中并提前加热至95-100℃(注意盖好以防液体蒸发),然后将切片放入修复盒中,在沸水浴环境中保持外沸状态15 min,室温自然冷却;用PBS缓冲液(试剂1,将干粉溶解在2L蒸馏水中)清洗5 min,重复3次。

3. 阻断内源性过氧化物酶

用吸水纸吸去玻片上多余的液体,用免疫组化笔在组织周围画圈,加入2-4内源性过氧化物酶阻断剂(试剂3,室温下置于湿盒中孵育15 min,用PBS洗涤5 min,重复3次。

4. 血清封闭

用吸水纸吸去玻片上多余的液体,加入2-4封闭工作液(试剂4,置于湿盒内37℃封闭20 min,以减少非特异性染色。

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