GLP-1单克隆即用型免疫组化试剂盒-分析方法-资讯-生物在线

GLP-1单克隆即用型免疫组化试剂盒

作者:上海信裕生物科技有限公司 2023-04-25T00:00 (访问量:237)

 GLP-1单克隆即用型免疫组化试剂盒

英文名称 GLP-1 Ready-To-Use IHC Kit
中文名称 GLP-1单克隆即用型免疫组化试剂盒
别    名 GCG; GLP 1; glucagon; Glucagon like peptide 1; GRPP; GLP-1(7-36); GLP-1(7-37); Oxyntomodulin; OXM; OXY; GLUC_HUMAN.   
种    属 Human, Mouse, Rat
应用范围 Immunohistochemistry
适用样品基质 PFPE tissue
描述 IHC0109 is a ready-to-use IHC kit for staining of GLP-1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
保存条件 Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍

 

 

 

产品组分及规格

编号

组分

规格

浓度

储存

1

PBS缓冲液干粉

2 L×2

20x

室温

2

抗原修复缓冲液

20 ml

100x

2-8

3

内源性过氧化物酶阻断剂

3 ml

RTU

2-8

4

封闭工作液

3 ml

RTU

2-8

5

一抗GLP-1 Mouse mAb

6 ml

RTU

2-8

6

二抗HRP-Goat anti-Mouse IgG pAb

6 ml

RTU

2-8

7

DAB kit20×)显色液

0.3 ml

RTU

-20℃

8

DAB kit20×)稀释液

0.3 ml

RTU

-20

9

复染试剂

5 ml

RTU

室温

10

封片剂

5 ml

RTU

室温

11

对照大鼠胰腺,小鼠胰腺,人胰腺

3

RTU

室温

12

说明书

1

 

 

 

背景

GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue.It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.


 

 

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次,每次15 min;依次置于不同浓度(100%95%90%80%70%)乙醇浸泡5 min,再置于蒸馏水洗涤5 min,重复3次。

2. 抗原修复

沸水浴修复:将100×抗原修复缓冲液(试剂2

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