NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。
研究使用平台：NMT重金属创新科研平台
期刊：Environmental Pollution
主题：硅藻的[Si依赖性Cd/ Cu/ Pb耐受力]的机制
标题：Mechanisms underlying silicon-dependent metal tolerance in the marine diatom Phaeodactylum tricornutum
影响因子：5.714
检测指标：Cd2+, Cu2+, Pb2+流速
检测样品：硅藻
Cd2+, Cu2+, Pb2+流实验处理方法：
硅藻细胞在低硅（46μM）和高硅（260μM）中生长
Cd2+, Cu2+, Pb2+流实验测试液成份：
10μM CdCl2，0.1 mM KCl, 0.1 mM MgCl2, 0.5 mM NaCl,0.3 mM MES, 0.2 mM Na2SO4, and 0.1% 蔗糖， pH 8.0
作者：深圳大学潘科、周贝贝
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中文摘要（谷歌机翻）
人为活动显着改变了沿海水域的化学计量和养分浓度。由于氮，磷和硅酸盐的投入不成比例，硅已成为潜在的限制养分。
营养物质比率的破坏会导致海洋硅藻中金属敏感性的变化和积累，海洋硅藻是需要硅才能生长的重要的一组真核浮游植物。
在这项研究中，我们检查了硅可用性对硅藻P. tricornutum中金属敏感性的影响。我们发现硅饥饿严重损害了其镉，铜和铅的耐受性。有趣的是，多条证据表明，富硅细胞比缺硅细胞具有更高的金属吸附和流入速率。
然而，富含Si的细胞还具有更高的响应能力，可以通过膜和液泡金属转运蛋白的表达增加以及更大的抗氧化活性来抵抗金属毒性，这些抗氧化活性可以清除金属应力产生的活性氧。
Fig. 3. Metal fluxes around the cell surface of individual P. tricornutum diatom. (A–C) Instantaneous net metal flux as measured by a non-invasive microelectrode probe. H-Si, high Si growth conditions; L-Si, low Si growth conditions; (D) the calculated mean net metal flux for the L-Si and H-Si cells. Asterisks indicate the L-Si and H-Si treatments significantly differ (***p ≤ 0.001).
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英文摘要
Anthropogenic activities have significantly changed the stoichiometry and concentrations of nutrients in coastal waters.Silicon (Si) has become a potential limiting nutrient due to disproportionate nitrogen, phosphorus, and silicate inputs into these areas.
The disrupted nutrient ratios can cause changes to metal sensitivity and accumulation in marine diatoms, an important group of eukaryotic phytoplankton that requires silicon for growth.
In this study, we examined the effects of Si availability on the metal sensitivity in the diatom P. tricornutum. We found that Si starvation dramatically compromised its cadmium, copper, and lead tolerances. Interestingly, multiple lines of evidence indicated that Si-enriched cells had higher metal adsorption and influx rates than Si-starved cells.
Yet Si-enriched cells also had a greater ability to respond to and counteract metal toxicity via elevated expression of membrane and vacuolar metal transporters and greater antioxidant activities which scavenge reactive oxygen species created by metal stress.