脯氨酰4-羟化酶α多肽Ⅱ(P4Ha2)重组蛋白说明书

Recombinant Prolyl-4-Hydroxylase Alpha Polypeptide II (P4Ha2)  

[ PROPERTIES ]

Residues: Ser207~Glu520

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: O15460

Host: E. coli

Subcellular Location: Endoplasmic reticulum

lumen.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 39.9kDa

Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37ºC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

SQVL DYLSYAVFQL GDLHRALELT RRLLSLDPSH ERAGGNLRYF EQLLEEEREKTLTNQTEAEL ATPEGIYERP VDYLPERDVY ESLCRGEGVK LTPRRQKRLF CRYHHGNRAPQLLIAPFKEE DEWDSPHIVR YYDVMSDEEI ERIKEIAKPK LARATVRDPK TGVLTVASYRVSKSSWLEED DDPVVARVNR RMQHITGLTV KTAELLQVAN YGVGGQYEPH FDFSRNDERDTFKHLGTGNR VATFLNYMSD VEAGGATVFP DLGAAIWPKK GTAVFWYNLL RSGEGDYRTRHAACPVLVGC KWVSNKWFHE

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用