皮瓣结构特异性核酸内切酶(FEN1)重组蛋白说明书

Recombinant Flap Structure Specific Endonuclease 1 (FEN1) 

[ PROPERTIES ]

ource: Prokaryotic expression

Host: E.coli

Residues: Met1~Thr195

Tags: N-terminal His Tag

Subcellular Location: Nucleus, Mitochondrion

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% SKL, 5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.9

Predicted Molecular Mass: 25.2kDa

Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date underappropriate storage condition. 

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ] 

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用