SPP1 polyclonal antibody
产品名称： SPP1 polyclonal antibody
英文名称： SPP1 polyclonal antibody
产品编号： PAB10297
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of SPP1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to human SPP1.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 66, 32
- Reactivity:
- Dog, Human, Pig
- Specificity:
- This antibody recognizes the full-length osteopontin protein (which runs at 66 KDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 KDa MMP-cleaved C-fragment is recognized, but not the 40 KDa N-terminal fragment.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.1% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:20000-1:100000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:100-1:300)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- The immunology of Eta-1/osteopontin.
Weber GF, Cantor H.Cytokine Growth Factor Rev. 1996 Oct;7(3):241-8.
- 2.
- Osteopontin-induced modifications of cellular functions.
Denhardt DT, Lopez CA, Rollo EE, Hwang SM, An XR, Walther SE.Ann N Y Acad Sci. 1995 Apr 21;760:127-42.
- 3.
- Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells.
Denhardt DT, Chambers AF.J Cell Biochem. 1994 Sep;56(1):48-51.
- Applications
- Western Blot (Recombinant protein)
- Immunoblotting of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1:1,000 dilution to detect SPP1 by Western blot.
In lane 1, reactivity is shown against 250 ng of human SPP1.
Lane 2 shows reactivity of MMP-cleaved SPP1.
Use a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG for detection.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1 : 100-1 : 300 dilution to detect SPP1 by immunohistochemistry.
SPP1 is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells).
There is also weak staining of the extracellular matrix.
SPP1 is not expressed in breast tumor cells, and there is no staining of the breast cells in this section.
No antigen retrieval is required.
- Entrez GeneID:
- 6696
- Gene Name:
- SPP1
- Gene Alias:
- BNSP,BSPI,ETA-1,MGC110940,OPN
- Gene Description:
- secreted phosphoprotein 1
- Omim ID:
- 166490
- Gene Ontology:
- Hyperlink
- Other Designations:
- SPP1/CALPHA1 fusion,bone sialoprotein I,early T-lymphocyte activation 1,osteopontin,osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein,secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
- Interactome
- Related Disease
- Adenocarcinoma
- Alzheimer Disease
- Alzheimer disease
- Amyotrophic Lateral Sclerosis
- Amyotrophic lateral sclerosis
- Anoxia
- Aortic Aneurysm, Abdominal
- Arthritis, Rheumatoid
- Asthma
- Asthma
- Atherosclerosis
- Atherosclerosis
- Brain Infarction
- Brain Ischemia
- Calcinosis
- Carcinoma, Hepatocellular
- Cardiovascular Diseases
- Carotid Artery Diseases
- Cerebrovascular Disorders