CHEK2 polyclonal antibody
产品名称： CHEK2 polyclonal antibody
英文名称： CHEK2 polyclonal antibody
产品编号： PAB18147
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of CHEK2.
- Immunogen:
- A synthetic peptide corresponding to human CHEK2.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse, Rat
- Specificity:
- This antibody is specific to CHEK2.
- Form:
- Liquid
- Purification:
- Affinity purification
- Concentration:
- 1 mg/mL
- Storage Buffer:
- In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Promyelocytic leukemia activates Chk2 by mediating Chk2 autophosphorylation.
Yang S, Jeong JH, Brown AL, Lee CH, Pandolfi PP, Chung JH, Kim MK.J Biol Chem. 2006 Sep 8;281(36):26645-54. Epub 2006 Jul 11.
- 2.
- Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation.
Schwarz JK, Lovly CM, Piwnica-Worms H.Mol Cancer Res. 2003 Jun;1(8):598-609.
- 3.
- Chk2 is a tumor suppressor that regulates apoptosis in both an ataxia telangiectasia mutated (ATM)-dependent and an ATM-independent manner.
Hirao A, Cheung A, Duncan G, Girard PM, Elia AJ, Wakeham A, Okada H, Sarkissian T, Wong JA, Sakai T, De Stanchina E, Bristow RG, Suda T, Lowe SW, Jeggo PA, Elledge SJ, Mak TW.Mol Cell Biol. 2002 Sep;22(18):6521-32.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of extracts from Jurkat cells, using CHEK2 polyclonal antibody (Cat # PAB18147).
Peptide "+" means "peptide blocking".
- Entrez GeneID:
- 11200
- Protein Accession#:
- O96017
- Gene Name:
- CHEK2
- Gene Alias:
- CDS1,CHK2,HuCds1,LFS2,PP1425,RAD53
- Gene Description:
- CHK2 checkpoint homolog (S. pombe)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000028871,checkpoint-like protein CHK2,protein kinase CHK2,serine/threonine-protein kinase CHK2
- Gene Pathway
- Related Disease
- Adenocarcinoma
- Adenoma
- Ataxia telangiectasia
- Atherosclerosis
- Atherosclerosis
- Barrett Esophagus
- Brain Neoplasms
- Breast cancer
- Breast Neoplasms
- Breast Neoplasms, Male
- Carcinoma
- Carcinoma, Ductal
- Carcinoma, Ductal, Breast
- Carcinoma, Intraductal, Noninfiltrating
- Carcinoma, Lobular
- Carcinoma, Medullary
- Carcinoma, Non-Small-Cell Lung
- Carcinoma, Pancreatic Ductal
- Carcinoma, Squamous Cell