Recombinant Human Interleukin-12 (Human IL-12)
产品名称： Recombinant Human Interleukin-12 (Human IL-12)
英文名称： Recombinant Human Interleukin-12 (Human IL-12)
产品编号： 90111ES10
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:09:52
使用范围： null
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640 点击查看
- 传真 : 点击查看
- 邮箱 : lizizhuan@yeasen.com
- 二维码 : 点击查看
-
Product Information
Product Name
Cat#
Size
Price（¥）
Recombinant Human Interleukin-12 (Human IL-12)
90111ES10
10 μg
1855.00
90111ES60
100 μg
13615.00
90111ES76
500 μg
35395.00
Product Description
Interleukin-12 (IL-12) is a cytokine that has regulatory effects on T and natural killer (NK) cells and is composed of two disulfide-bonded subunits, p40 and p35. It is a heterodimeric cytokine produced by macrophages, mitogen stimulated- or EBV infected-B lymphocytes, keratinocytes, and probably dendritic cells, with important immunoregulatory functions in vitro and in vivo. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS. Importantly, IL-12 also inhibited secondary granuloma formation in mice presensitized with eggs demonstrating a role for the cytokine in reversing established Th2-type responses.
Product Properties
Synonyms
Interleukin-12, IL-12, NKSF2, CLMF p40
Accession
GeneID
Source
Sf21-derived human IL-12 protein.
Molecular Weight
Approximately 41 kDa and 29 kDa under reducing conditions.
AA Sequence
HuIL-12 p40: Ile23 - Ser328; Accession # P29460
HuIL-12 p35: Arg23 - Ser219; Accession # P29459Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97 % by SDS-PAGE.
Biological Activity
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rH IL-12 is approximately 1.1 × 104 units/μg, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544).
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriately buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!
HB220210