DNA指导聚合酶α1(POLα1)重组蛋白

YB713Mu012
Recombinant Polymerase DNA Directed Alpha 1 (POLa1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
DNA指导聚合酶α1(POLα1)重组蛋白[ PROPERTIES ]
Residues: Gly1240~Ser1462
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P09884
Host: E. coli
Subcellular Location: Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
DNA指导聚合酶α1(POLα1)重组蛋白Predicted isoelectric point: 7.6
Predicted Molecular Mass: 29.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
DNA指导聚合酶α1(POLα1)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
G LDPTQFRVHH YHKDEE NDA L LGGPAQ LTDE EKYRDCE RFK CPCPT CGTEN
IYDNVFDGSG TDMEPSLYRC SNIDCKASPL TFTVQLSNKL IMDIRRFIKK YYDGWLICEE
PTCRNRTRHL PLQFSRTGPL CPACMKATLQ PEYSDKSLYT QLCFYRYIFD AECALEKLTT
DHEKDKLKKQ FFTPKVLQDY RKLKNTAEQF LSRSGYSEVN LSKLFAGCAV KS