PSMD4 抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
PSMD4 抗体

PSMD4 抗体

商家询价

产品名称： PSMD4 抗体

英文名称： PSMD4 Antibody

产品编号： XY14899-1-

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 PSMD4 抗体Applications

In-house applications ELISA, WB, IHC, FC, IF     Application not tested? Please see our Guarantee.
Published applications IF, WB Publications 5 citations –view here
Species specificity Human,Mouse,Rat; other species not tested.
Published Species Human, mouse
Images
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  • 14899-1-AP;K-562 cellsK-562 cells were subjected to SDS PAGE followed by western blot with 14899-1-AP(PSMD4 antibody) at dilution of 1:3000
  • 14899-1-AP;human lung cancer tissueImmunohistochemistry of paraffin-embedded human lung cancer slide using 14899-1-AP( PSMD4 Antibody) at dilution of 1:50
  • 14899-1-AP;human lung cancer tissueImmunohistochemistry of paraffin-embedded human lung cancer slide using 14899-1-AP( PSMD4 Antibody) at dilution of 1:50
  • 14899-1-AP;HepG2 cellsImmunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 14899-1-AP( PSMD4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • 14899-1-AP;HepG2 cells1X10^6 HepG2 cells were stained with 0.2ug PSMD4 antibody (14899-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Positive WB detected in K-562 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, Raji cells, rat spleen tissue, RAW 264.7 cells
Positive IHC detected in Human lung cancer tissue, human testis tissue
Positive IF detected in HepG2 cells
Positive FC detected in HepG2 cells
Recommended dilution WB : 1:1000-1:10000
IHC : 1:20-1:200
IF : 1:20-1:200
 PSMD4 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen PSMD4 fusion protein ag6691 Full name Proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular
weight		 41 kDa Observed molecular 
weight		 50-55 kDa
GenBank accession 
number		 BC002365 Gene ID (NCBI) 5710
Gene symbol PSMD4 Synonyms AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A
Background PSMD4 抗体

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4. PSMD4 抗体

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