LCP2 monoclonal antibody, clone SLP-76/03
产品名称： LCP2 monoclonal antibody, clone SLP-76/03
英文名称： LCP2 monoclonal antibody, clone SLP-76/03
产品编号： MAB3647
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against partial recombinant LCP2.
- Immunogen:
- Recombinant His fusion protein corresponding to amino acids 216-434 of human LCP2.
- Host:
- Mouse
- Theoretical MW (kDa):
- 76
- Reactivity:
- Human, Mouse, Pig
- Specificity:
- The polyclonal antibody reacts with LCP2, a 76KDa cytosolic adaptor protein that isinvolved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by LCP2.
- Form:
- Liquid
- Concentration:
- 1 mg/mL
- Isotype:
- IgG2b
- Storage Buffer:
- In PBS, pH 7.4 (15 mM sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- SLP-76 mediates and maintains activation of the Tec family kinase ITK via the T cell antigen receptor-induced association between SLP-76 and ITK.
Bogin Y, Ainey C, Beach D, Yablonski D.Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6638-43. Epub 2007 Apr 9.
- 2.
- Dual role of SLP-76 in mediating T cell receptor-induced activation of phospholipase C-gamma1.
Beach D, Gonen R, Bogin Y, Reischl IG, Yablonski D.J Biol Chem. 2007 Feb 2;282(5):2937-46. Epub 2006 Dec 4.
- 3.
- SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond.
Koretzky GA, Abtahian F, Silverman MA.Nat Rev Immunol. 2006 Jan;6(1):67-78.
- Applications
- Western Blot (Cell lysate)
- Western Blotting analysis (reducing conditions) of whole cell lysate using LCP2 monoclonal antibody, clone SLP-76/03 (Cat # MAB3647).
Lane 1 : HPB-ALL (human peripheral blood T cell leukemia cell line).
Lane 2 : Jurkat (human peripheral blood T cell leukemia cell line).
Lane 3 : RAMOS (human Burkitt lymphoma cell line).
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Entrez GeneID:
- 3937
- Gene Name:
- LCP2
- Gene Alias:
- SLP-76,SLP76
- Gene Description:
- lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
- Omim ID:
- 601603
- Gene Ontology:
- Hyperlink
- Gene Summary:
- SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function. [provided by RefSeq
- Other Designations:
- 76 kDa tyrosine phosphoprotein,SH2 domain-containing leukocyte protein of 76kD,lymphocyte cytosolic protein 2,lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76kD)
- Related Disease