DGKE (N-term) Peptide
产品名称： DGKE (N-term) Peptide
英文名称： DGKE (N-term) Peptide
产品编号： P3130
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DGKE.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DGKE.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Characterization of the human diacylglycerol kinase epsilon gene and its assessment as a candidate for inherited retinitis pigmentosa.
Tang W, Bardien S, Bhattacharya SS, Prescott SM.Gene. 1999 Oct 18;239(1):185-92.
- 2.
- Cytogenetic assignment and physical mapping of the human DGKE gene to chromosome 17q22.
Hart TC, Price JA, Bobby PL, Pettenati MJ, Shashi V, Von Kap Herr C, Van Dyke TE.Genomics. 1999 Mar 1;56(2):233-5.
- 3.
- Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates.
Tang W, Bunting M, Zimmerman GA, McIntyre TM, Prescott SM.J Biol Chem. 1996 Apr 26;271(17):10237-41.
- Applications
- Western Blot (Cell lysate)
- The DGKE polyclonal antibody (Cat # PAB3344) is used in Western blot to detect DGKE in Jurkat cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKE polyclonal antibody (Cat # PAB3344) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 8526
- Protein Accession#:
- P52429
- Gene Name:
- DGKE
- Gene Alias:
- DAGK6,DGK
- Gene Description:
- diacylglycerol kinase, epsilon 64kDa
- Omim ID:
- 601440
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq
- Other Designations:
- diacylglycerol kinase epsilon,diacylglycerol kinase, epsilon (64kD)
- Related Disease