NEDD8 (N-term) Peptide
产品名称： NEDD8 (N-term) Peptide
英文名称： NEDD8 (N-term) Peptide
产品编号： P0290
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of NEDD8.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NEDD8.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.
Bohnsack RN, Haas AL.J Biol Chem. 2003 Jul 18;278(29):26823-30. Epub 2003 May 10.
- 2.
- DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1.
Wu K, Yamoah K, Dolios G, Gan-Erdene T, Tan P, Chen A, Lee CG, Wei N, Wilkinson KD, Wang R, Pan ZQ.J Biol Chem. 2003 Aug 1;278(31):28882-91. Epub 2003 May 19.
- 3.
- The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast cancer cells.
Fan M, Bigsby RM, Nephew KP.Mol Endocrinol. 2003 Mar;17(3):356-65. Epub 2002 Dec 18.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of NEDD8 polyclonal antibody (Cat # PAB1737) in HeLa cell lysate. NEDD8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEDD8 polyclonal antibody (Cat # PAB1737) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.