MAP4K5 monoclonal antibody (M01), clone 3H3
产品名称： MAP4K5 monoclonal antibody (M01), clone 3H3
英文名称： MAP4K5 monoclonal antibody (M01), clone 3H3
产品编号： H00011183-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant MAP4K5.
- Immunogen:
- MAP4K5 (AAH36013, 361 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- SSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLP
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody (M01), clone 3H3.
Lane 1: MAP4K5 transfected lysate(95.04 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged MAP4K5 is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi ( Cat # H00011183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody (M01) clone 3H3 (Cat # H00011183-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 11183
- GeneBank Accession#:
- BC036013
- Protein Accession#:
- AAH36013
- Gene Name:
- MAP4K5
- Gene Alias:
- GCKR,KHS,KHS1,MAPKKKK5
- Gene Description:
- mitogen-activated protein kinase kinase kinase kinase 5
- Omim ID:
- 604923
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq
- Other Designations:
- germinal center kinase-related,kinase homologous to SPS1/STE20
- Related Disease