
pMDC123-2x35S-Cas9-Nos-gRNA植物CRISPR/Cas9载体质粒
产品名称: pMDC123-2x35S-Cas9-Nos-gRNA植物CRISPR/Cas9载体质粒
英文名称: pMDC123-2x35S-Cas9-Nos-gRNA植物CRISPR/Cas9载体质粒粒
产品编号: BiovectorPMDCCN
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
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Order ID |
Name |
Description |
BiovectorPMDCCN |
pMDC123-2x35S-Cas9-Nos-gRNA |
pMDC123-2x35S-Cas9-Nos-gRNA 15uL DNA. AmpR.Strage:-20℃ |
pMDC123-2x35S-Cas9-Nos-gRNA植物CRISPR/Cas9载体质粒图谱plant CRISPR-Cas9 vector
Standard heat-shock transformation of chem ically compe tent bacteria
- Take competent cells out of -80°C and thaw on ice.
- Take agar plates (containing the appropriate antibiotic) out of 4°C to warm up to room temperature or place in 37°C incubator.
- Mix 5μl of DNA into 100μL competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times.
- Place the competent cell/DNA mixture on ice for 20-30min.
- Heat shock each transformation tube by placing the bottom 2/3 of the tube into a 42°C water bath for 42 seconds. Put the tubes back on ice for 2 min.
- Add 250-500μl SOC media (without antibiotic) and grow in 37°C shaking incubator for 45min.