GAB1 monoclonal antibody, clone 1AT1979
产品名称： GAB1 monoclonal antibody, clone 1AT1979
英文名称： GAB1 monoclonal antibody, clone 1AT1979
产品编号： MAB1139
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant GAB1.
- Immunogen:
- Recombinant GST fusion protein corresponding to full length human GAB1.
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Isotype:
- IgG1
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:200-1000)
Immuniprecipitation (2-5 ug)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Grb2-associated binder 1 (Gab1) genetic polymorphism, Helicobacter pylori infection, and chronic atrophic gastritis among older adults from Germany.
Gao L, Weck MN, Nieters A, Brenner H.Mol Carcinog. 2010 Oct;49(10):869-73.
- 2.
- An approach based on a genome-wide association study reveals candidate loci for narcolepsy.
Shimada M, Miyagawa T, Kawashima M, Tanaka S, Honda Y, Honda M, Tokunaga K.Hum Genet. 2010 Oct;128(4):433-41. Epub 2010 Jul 31.
- 3.
- Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S.Diabetes Care. 2010 Oct;33(10):2250-3. Epub 2010 Jul 13.
- Applications
- Western Blot
- Rescue of the JNK pathway by expression of wild-type GAB1 in GAB1-/- cells. GAB1-/- cells were transiently transfected with 1, 2, or 4 ug of the expression construct for human GAB1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of GAB1 in transfected cells was confirmed by anti-GAB1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, GAB1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24 (4) :1531-1539)
- Immunoprecipitation
- Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42 °C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with GAB1 monoclonal antibody, clone 1AT1979 (Cat # MAB1139) . Data courtesy of Dr. GS Feng at The Burnham Institute.
- Entrez GeneID:
- 2549
- Protein Accession#:
- NP_997006;Q13480
- Gene Name:
- GAB1
- Gene Alias:
- -
- Gene Description:
- GRB2-associated binding protein 1
- Omim ID:
- 604439
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- -
- Interactome
